September 9, 2024 Christopher Hall President and Chief Executive Officer Personalis, Inc. 6600 Dumbarton Circle Fremont, CA, 94555 Re: Personalis, Inc. Registration Statement on Form S-3 Filed August 30, 2024 File No. 333-281886 Dear Christopher Hall: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Robert Augustin at 202-551-8483 with any questions. Sincerely, Division of Corporation Finance Office of Industrial Applications and Services cc: Laura Berezin